The protocol below was taken from Illumina’s recommendations

PCR Enrichment of DSN Normalized Library

  1. Prepare the following PCR reaction mix in a separate, sterile, nuclease‐free tube for each of the samples being prepared. Mix well by pipetting up and down several times.
Component Volume
DSN Treated Library 30 ul
2X KAPA HiFi HotStart ReadyMix 25 ul
10X KAPA Library Amplification Primer Mix 5 ul
Total Volume per sample 50 ul
  1. Amplify the library using the following thermal cycling protocol:
Step Temp Duration Cycles
Initial denaturation 98 °C 45 sec 1
Denaturation 98 °C 15 sec 12
Annealing 60 °C 30 sec 12
Extension 72 °C 30 sec 12
Final Extension 72 °C 5 min 1
Hold 10 °C ∞ 1
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