laboratory

Laboratory Work

This repository holds the methods we used for sequencing library preparation ~2019.

Suggested Use

Our protocols change over time. If you want to create a snapshot of your method at the time you ran the protocol, create a release. This will create an archive of the method that you can refer back to years later when you want to share a tip with someone or reference your work in a manuscript.

ddRADSeq

This is used in our Amphiprion clarkii clownfish project and our Paralichthys dentatus summer flounder project.

Nextera

This method is used in our Myotis lucificus little brown bat project.

EecSeq

This is the expresded exome capture sequencing method used in Amphiprion percula larval trait GWAS study by Katrina Calatano ~2021.

protocols

Ala carte menu of procedures used in the lab.

Michelle Stuart Amphiprion clarkii Lab Notebooks 2014-2020

Documenting each day’s lab work and connecting sample IDs to extraction, digest, and ligation IDs for sequencing. For bioinformatcs on these samples, see the genomics repo