This recipe is the same for ddRADSeq and EecSeq protocols and will prepare a 10 mL solution.
annealing stock solution |
|---|
| item |
initial_conc |
final_conc |
final_vol_uL |
initial_vol_uL |
| 500_mM_edta_ul |
500 |
10 |
5000 |
100.0000 |
| 1000_mM_Tris-HCl_pH8_ul |
1000 |
100 |
5000 |
500.0000 |
| 4.45_M_NaCl_ul |
4450 |
500 |
5000 |
561.7978 |
| ph2o_ul |
0 |
0 |
5000 |
3838.2022 |
rm(annealing_guide)
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