For this plate, the original loading plan was abandoned and APCL14_303 was loaded into F1 instead of APCL13_048.  This shifted all of the samples by 1 well


 1 2 3 4 5 6 7 8 9 10 11 12
A L1591 L1599 L1607 L1615 L1623 L1631 L1639 L1647 L1655 L1663 L1671 L1679
B L1592 L1600 L1608 L1616 L1624 L1632 L1640 L1648 L1656 L1664 L1672 L1680
C L1593 L1601 L1609 L1617 L1625 L1633 L1641 L1649 L1657 L1665 L1673 L1681
D L1594 L1602 L1610 L1618 L1626 L1634 L1642 L1650 L1658 L1666 L1674 L1682
E L1595 L1603 L1611 L1619 L1627 L1635 L1643 L1651 L1659 L1667 L1675 L1683
F L1596 L1604 L1612 L1620 L1628 L1636 L1644 L1652 L1660 L1668 L1676 L1684
G L1597 L1605 L1613 L1621 L1629 L1637 L1645 L1653 L1661 L1669 L1677 L1685
H L1598 L1606 L1614 L1622 L1630 L1638 L1646 L1654 L1662 L1670 L1678 L1686

L1607 is APCL13_059 which 

Google docs plate map:

 1 2 3 4 5 6 7 8 9 10 11 12
A D0370 4.1 21.1 D0390 3.2 18.7 D0399 2.5 20.6 D0427 1.0 18.5 D0533 5.4 17.1 D0637 6.5 17.7 D0372 1.9 20.3 D0414 1.3 20.9 D0429 2.6 19.6 D0660 2.7 19.5 D0738 7.8 14.4 D1205 3.5 18.7
B D0371 4.6 21.5 D0391 3.2 19.9 D0401 4.0 20.9 D0433 4.7 21.1 D0535 4.5 17.6 D0676 2.9 18.2 D0373 1.3 20.9 D0415 1.2 21.0 D0430 1.9 20.3 D0663 7.3 14.9 D0759 1.7 20.5 D1211 7.1 15.1
C D0374 3.4 20.1 D0392 3.0 20.4 D0403 7.3 20.7 D0438 6.5 16.0 D0543 1.4 17.0 D0684 3.1 11.9 D0400 1.3 20.9 D0416 1.3 20.9 D0435 1.1 21.1 D0703 4.5 17.7 D0760 1.7 20.5 D1213 7.8 14.4
D D0376 3.8 19.9 D0393 3.0 18.9 D0406 3.6 20.8 D0513 4.1 20.4 D0545 2.2 17.4 D0700 6.2 14.7 D0402 1.6 20.6 D0417 1.2 21.0 D0436 1.4 20.8 D0705 2.8 19.4 D0762 4.2 18.0 D1422 5.1 17.1
E D0385 7.1 14.7 D0394 3.2 20.6 D0408 3.4 20.9 D0516 4.3 20.5 D0546 2.8 19.0 D0725 7.0 19.9 D0478 9.2 13.0 D0693 9.8 12.4 D0688 9.7 12.5 D0729 9.3 12.9 D0770 0.9 21.3 D1463 5.5 16.7
F D0386 4.6 16.9 D0734 6.9 15.3 D0410 5.2 16.9 D0518 4.8 17.0 D0572 1.5 14.6 D0765 5.5 17.7 D0405 1.6 20.6 D0422 1.8 20.4 D0520 6.2 16.0 D0721 2.3 19.9 D0797 4.0 18.2 D1464 3.0 19.2
G D0388 3.5 20.5 D0396 3.1 20.7 D0418 6.4 20.2 D0524 2.1 19.6 D0613 2.7 20.7 D0766 6.0 16.5 D0409 1.3 20.9 D0423 1.1 21.1 D0522 1.2 21.0 D0731 6.2 16.0 D1467 7.8 14.4 D1465 4.1 18.1
H D0389 6.7 20.3 D0397 2.8 20.7 D0421 3.0 16.0 D0525 6.9 14.2 D0616 2.9 15.0 D1387 5.0 18.9 D0413 2.0 20.2 D0424 1.0 21.2 D0526 4.6 17.6 D0736 6.0 16.2 D1203 7.9 14.3 D1466 6.4 15.8













Ligation_ID's for ligation plate 1 (200ng/25ng)












1 2 3 4 5 6 7 8 9 10 11 12
A APCL13_032L1591 APCL13_052L1599 APCL13_061L1607 APCL13_089L1615 APCL13_196L1623 APCL13_301L1631 APCL13_034L1639 APCL13_076L1647 APCL13_091L1655 APCL13_324L1663 APCL13_402L1671 APCL14_502L1679
B APCL13_033L1592 APCL13_053L1600 APCL13_063L1608 APCL13_095L1616 APCL13_199L1624 APCL13_340L1632 APCL13_035L1640 APCL13_077L1648 APCL13_092L1656 APCL13_327L1664 APCL13_002L1672 APCL14_520L1680
C APCL13_036L1593 APCL13_054L1601 APCL13_065L1609 APCL13_100L1617 APCL13_207L1625 APCL13_348L1633 APCL13_062L1641 APCL13_078L1649 APCL13_097L1657 APCL13_367L1665 APCL13_003L1673 APCL14_524L1681
D APCL13_038L1594 APCL13_055L1602 APCL13_068L1610 APCL13_176L1618 APCL13_209L1626 APCL13_364L1634 APCL13_064L1642 APCL13_079L1650 APCL13_098L1658 APCL13_369L1666 APCL13_426L1674 APCL14_517L1682
E APCL13_047L1595 APCL13_056L1603 APCL13_070L1611 APCL13_179L1619 APCL13_210L1627 APCL13_389L1635 APCL13_140L1643 APCL13_357L1651 APCL13_352L1659 APCL13_393L1667 APCL13_005L1675 APCL14_466L1683
F APCL13_048L1596 APCL13_398L1604 APCL13_072L1612 APCL13_181L1620 APCL13_236L1628 APCL13_429L1636 APCL13_067L1644 APCL13_084L1652 APCL13_183L1660 APCL13_385L1668 APCL13_461L1676 APCL14_469L1684
G APCL13_050L1597 APCL13_058L1605 APCL13_080L1613 APCL13_187L1621 APCL13_277L1629 APCL13_430L1637 APCL13_071L1645 APCL13_085L1653 APCL13_185L1661 APCL13_395L1669 APCL14_489L1677 APCL14_471L1685
H APCL13_051L1598 APCL13_059L1606 APCL13_083L1614 APCL13_188L1622 APCL13_280L1630 APCL14_303L1638 APCL13_075L1646 APCL13_086L1654 APCL13_189L1662 APCL13_400L1670 APCL14_499L1678 APCL14_477L1686














1 2 3 4 5 6 7 8 9 10 11 12
A APCL13_032L1591 APCL13_051L1599 APCL13_059L1607 APCL13_083L1615 APCL13_188L1623 APCL13_280L1631 APCL13_034L1639 APCL13_075L1647 APCL13_086L1655 APCL13_189L1663 APCL13_400L1671 APCL14_499L1679
B APCL13_033L1592 APCL13_052L1600 APCL13_061L1608 APCL13_089L1616 APCL13_196L1624 APCL13_301L1632 APCL13_035L1640 APCL13_076L1648 APCL13_091L1656 APCL13_324L1664 APCL13_402L1672 APCL14_502L1680
C APCL13_036L1593 APCL13_053L1601 APCL13_063L1609 APCL13_095L1617 APCL13_199L1625 APCL13_340L1633 APCL13_062L1641 APCL13_077L1649 APCL13_092L1657 APCL13_327L1665 APCL13_002L1673 APCL14_520L1681
D APCL13_038L1594 APCL13_054L1602 APCL13_065L1610 APCL13_100L1618 APCL13_207L1626 APCL13_348L1634 APCL13_064L1642 APCL13_078L1650 APCL13_097L1658 APCL13_367L1666 APCL13_003L1674 APCL14_524L1682
E APCL13_047L1595 APCL13_055L1603 APCL13_068L1611 APCL13_176L1619 APCL13_208 or 209 APCL13_364L1635 L1643 APCL13_079L1651 L1659 APCL13_393L1667 APCL13_426L1675 APCL14_517L1683
F APCL14_303L1596 APCL13_056L1604 APCL13_070L1612 APCL13_179L1620 APCL13_210L1628 APCL13_389L1636 L1644 APCL13_357L1652 APCL13_352L1660 APCL13_393L1668 APCL13_005L1676 APCL14_466L1684
G APCL13_048L1597 APCL13_398L1605 APCL13_072L1613 APCL13_181L1621 APCL13_236L1629 APCL13_429L1637 APCL13_067L1645 APCL13_084L1653 APCL13_183L1661 APCL13_385L1669 APCL13_461L1677 APCL14_469L1685
H APCL13_050L1598 APCL13_058L1606 APCL13_080L1614 APCL13_187L1622 APCL13_277L1630 APCL13_430L1638 APCL13_071L1646 APCL13_085L1654 APCL13_185L1662 APCL13_395L1670 APCL14_489L1678 APCL14_471L1686
















didn't seq well enough to evaulate











offset of 1











not offset of 1, not followng pattern











original well is correct well, not following pattern











more complicated explanation - see lab notebook











have to check in R on work computer











L1659 does not match 098











L1627 matches 208 and 209











L1667 could be 367 or 393, matches recapture 14_357











APCL13_189/185 false matches APCL13_057









Original evernote plate map:
Ligation_ID's for ligation plate 1 (200ng/25ng)

 1 2 3 4 5 6 7 8 9 10 11 12
A APCL13_032L1591 APCL13_052L1599 APCL13_061L1607 APCL13_089L1615 APCL13_196L1623 APCL13_301L1631 APCL13_034L1639 APCL13_076L1647 APCL13_091L1655 APCL13_324L1663 APCL13_402L1671 APCL14_502L1679
B APCL13_033L1592 APCL13_053L1600 APCL13_063L1608 APCL13_095L1616 APCL13_199L1624 APCL13_340L1632 APCL13_035L1640 APCL13_077L1648 APCL13_092L1656 APCL13_327L1664 APCL13_002L1672 APCL14_520L1680
C APCL13_036L1593 APCL13_054L1601 APCL13_065L1609 APCL13_100L1617 APCL13_207L1625 APCL13_348L1633 APCL13_062L1641 APCL13_078L1649 APCL13_097L1657 APCL13_367L1665 APCL13_003L1673 APCL14_524L1681
D APCL13_038L1594 APCL13_055L1602 APCL13_068L1610 APCL13_176L1618 APCL13_209L1626 APCL13_364L1634 APCL13_064L1642 APCL13_079L1650 APCL13_098L1658 APCL13_369L1666 APCL13_426L1674 APCL14_517L1682
E APCL13_047L1595 APCL13_056L1603 APCL13_070L1611 APCL13_179L1619 APCL13_210L1627 APCL13_389L1635 APCL13_140L1643 APCL13_357L1651 APCL13_352L1659 APCL13_393L1667 APCL13_005L1675 APCL14_466L1683
F APCL13_048L1596 APCL13_398L1604 APCL13_072L1612 APCL13_181L1620 APCL13_236L1628 APCL13_429L1636 APCL13_067L1644 APCL13_084L1652 APCL13_183L1660 APCL13_385L1668 APCL13_461L1676 APCL14_469L1684
G APCL13_050L1597 APCL13_058L1605 APCL13_080L1613 APCL13_187L1621 APCL13_277L1629 APCL13_430L1637 APCL13_071L1645 APCL13_085L1653 APCL13_185L1661 APCL13_395L1669 APCL14_489L1677 APCL14_471L1685
H APCL13_051L1598 APCL13_059L1606 APCL13_083L1614 APCL13_188L1622 APCL13_280L1630 APCL14_303L1638 APCL13_075L1646 APCL13_086L1654 APCL13_189L1662 APCL13_400L1670 APCL14_499L1678 APCL14_477L1686