extracted - gelled - quantified - pulled for digest

The formula to make this lysis buffer was missing 224 uL of proteinate K (had 4mL of it, just not the .2mL)

sample Map of tray 1:
               1
                     2
                      3
                  4
                  5
                 6
                  7
           8
       9
     10
     11
        12

























A
APCL16_189
APCL16_197
APCL16_204
APCL16_212
APCL16_220
APCL16_228
APCL16_236
APCL16_244
APCL16_251
APCL16_259
APCL16_267
APCL16_275
B
APCL16_190
APCL16_198
APCL16_205
APCL16_213
APCL16_221
APCL16_229
APCL16_237
APCL16_245
APCL16_252
APCL16_260
APCL16_268
APCL16_276
C
APCL16_191
APCL16_199
APCL16_206
APCL16_214
APCL16_222
APCL16_230
APCL16_238
APCL16_246
APCL16_253
APCL16_261
APCL16_269
APCL16_277
D
APCL16_192
XXXX
APCL16_207
APCL16_215
APCL16_223
APCL16_231
APCL16_239
APCL16_247
APCL16_254
APCL16_262
APCL16_270
APCL16_278
E
APCL16_193
APCL16_200
APCL16_208
APCL16_216
APCL16_224
APCL16_232
APCL16_240
XXXX
APCL16_255
APCL16_263
APCL16_271
APCL16_279
F
APCL16_194
APCL16_201
APCL16_209
APCL16_217
APCL16_225
APCL16_233
APCL16_241
APCL16_248
APCL16_256
APCL16_264
APCL16_272
APCL16_280
G
APCL16_195
APCL16_202
APCL16_210
APCL16_218
APCL16_226
APCL16_234
APCL16_242
APCL16_249
APCL16_257
APCL16_265
APCL16_273
APCL16_281
H
APCL16_196
APCL16_203
APCL16_211
APCL16_219
APCL16_227
APCL16_235
APCL16_243
APCL16_250
APCL16_258
APCL16_266
APCL16_274
APCL16_282

extraction ids
         1
        2
        3
        4
       5
       6
        7
          8
       9
     10
     11
        12

























A
E3161
E3169
E3176
E3184
E3192
E3200
E3208
E3216
E3223
E3231
E3239
E3247
B
E3162
E3170
E3177
E3185
E3193
E3201
E3209
E3217
E3224
E3232
E3240
E3248
C
E3163
E3171
E3178
E3186
E3194
E3202
E3210
E3218
E3225
E3233
E3241
E3249
D
E3164
E4383
E3179
E3187
E3195
E3203
E3211
E3219
E3226
E3234
E3242
E3250
E
E3165
E3172
E3180
E3188
E3196
E3204
E3212
E4384
E3227
E3235
E3243
E3251
F
E3166
E3173
E3181
E3189
E3197
E3205
E3213
E3220
E3228
E3236
E3244
E3252
G
E3167
E3174
E3182
E3190
E3198
E3206
E3214
E3221
E3229
E3237
E3245
E3253
H
E3168
E3175
E3183
E3191
E3199
E3207
E3215
E3222
E3230
E3238
E3246
E3254
 The purple wells are blanks

Gel was loaded with the 12-channel pipet 





> (row1a <- set1$extraction_id)
[1] "E3161" "E3162" "E3169" "E3170" "E3176" "E3177" "E3184" "E3185" "E3192" "E3193" "E3200" "E3201" "E3208"  "E3209" "E3216" "E3217" "E3223" "E3224" "E3231" "E3232" "E3239" "E3240" "E3247" “E3248"

Ladder

> (row1b <- set2$extraction_id)
[1] "E3163" "E3164" "E3171" "E4383" "E3178" "E3179" "E3186" "E3187" "E3194" "E3195" "E3202" "E3203" "E3210"
[14] "E3211" "E3218" "E3219" "E3225" "E3226" "E3233" "E3234" "E3241" "E3242" "E3249" "E3250"

Ladder

> (row2a <- set3$extraction_id)
[1] "E3165" "E3166" "E3172" "E3173" "E3180" "E3181" "E3188" "E3189" "E3196" "E3197" "E3204" "E3205" "E3212"
[14] "E3213" "E4384" "E3220" "E3227" "E3228" "E3235" "E3236" "E3243" "E3244" "E3251" "E3252"

Ladder

> (row2b <- set4$extraction_id)
[1] "E3167" "E3168" "E3174" "E3175" "E3182" "E3183" "E3190" "E3191" "E3198" "E3199" "E3206" "E3207" "E3214"
[14] "E3215" "E3221" "E3222" "E3229" "E3230" "E3237" "E3238" "E3245" "E3246" "E3253" "E3254"
>

Ladder

All of the samples on plate E3161-E3254 had bands