Pull for low level digest (some of these have never been digested)
E2969 | E3002 | E3007 | E3010 | E3015 | E3016 | E3018 | E3020 | E3021 | E3022 | E3023 | E3024 | E3025 | E3026 | E3027 | E3029 | E3030 | E3031 | E3032 | E3034 | E3035 | E3036 | E3037 | E3038 | E3039 | E3042 | E3044 | E3045 | E3047 | E3048 | E3050 | E3051 | E3055 | E3056 |
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E2969 failed to sequence - Questionable on the gel, should have had enough DNA but maybe should not have been ligated at 200 - try 10 instead. - redigest at low conc
E3002 failed to sequence - Looks good on the gel, should have had enough DNA but maybe should not have been ligated at 200 - try 10 instead. - redigest
These samples need low conc digest
"E2968" "E2969" "E2976" "E2977" "E3010" "E3018" "E3019" "E3026" "E3051"
"E3059" "E3014" "E3030" "E3047" “E3062"
The entire E-F section didn’t work - I think this should be re-gelled on a small gel before proceeding
Smears
"E3041" "E3048" E2970 E2971 “E3054"
extracted - gelled - quantified - pulled for digest
source: