pull for low level digest:
E2572
E2583
E2602
E2607
E2630


E2630 failed to sequence  - no gel, very low concentration,  should ligate at 10ng - redigest low conc (has not been loaded into redigest)

E2607 - no gel, very low concentration - redigest low conc (has not been loaded into redigest)

E2602 - no gel, very low concentration - redigest low conc (has not been loaded into redigest)

E2572 - no gel, very low concentration - redigest low conc (has not been loaded into redigest)

E2548 - no gel, should have had plenty of DNA to successfully sequence - should ligate at 10ng - has been loaded for redigest plate 7


1
2
3
4
5
6
7
8
9
10
11
12
A
E2546
E2554
E2562
E2570
E2578
E2586
E2594
E2602
E2610
E2618
E2626
E2634
B
E2547
E2555
E2563
E2571
E2579
E2587
E2595
E2603
E2611
E2619
E2627
E2635
C
E2548
E2556
E2564
E2572
E2580
E2588
E2596
E2604
E2612
E2620
E2628
E2636
D
E2549
E2557
E2565
E2573
E2581
E2589
E2597
E2605
E2613
E2621
E2629
E2637
E
E2550
E2558
E2566
E2574
E2582
E2590
E2598
E2606
E2614
E2622
E2630
E2638
F
E2551
E2559
E2567
E2575
E2583
E2591
E2599
E2607
E2615
E2623
E2631
E2639
G
E2552
E2560
E2568
E2576
E2584
E2592
E2600
E2608
E2616
E2624
E2632
E2640
H
E2553
E2561
E2569
E2577
E2585
E2593
E2601
E2609
E2617
E2625
E2633
E2641


E2617 is APCL15_355704 from Magbangon, matches to APCL14_558 - captured in Palanas.  I’m leaning toward the error being on the 2014 fish but there have been some strange 2015_05 errors, so I can’t rule this out.  Would need to see more errors from 2015_05 to narrow down what is going on.

Original map in evernote from 2016-02-19 Friday:

1
2
3
4
5
6
7
8
9
10
11
12
A
APCL15_369218E2546
APCL15_090E2554
APCL15_373231E2562
APCL15_356778E2570
APCL15_041E2578
APCL15_403892E2586
APCL15_356001E2594
APCL15_353914E2602
APCL15_370062E2610
APCL15_367983E2618
APCL15_030E2626
APCL15_028E2634
B
APCL15_370326E2547
APCL15_404452E2555
APCL15_354270E2563
APCL15_067E2571
APCL15_3733511E2579
APCL15_403913E2587
APCL15_033E2595
APCL15_354587E2603
APCL15_070E2611
APCL15_354641E2619
APCL15_372081E2627
APCL15_376036E2635
C
APCL15_401739E2548
APCL15_093E2556
XXXX_E2564
APCL15_355518E2572
APCL15_039E2580
APCL15_402724E2588
APCL15_038E2596
APCL15_027E2604
APCL15_370215E2612
APCL15_355054E2620
APCL15_363708E2628
APCL15_354327E2636
D
APCL15_356312E2549
APCL15_354523E2557
APCL15_355444E2565
APCL15_063E2573
APCL15_372487E2581
APCL15_042E2589
APCL15_406954E2597
APCL15_399642E2605
APCL15_373816E2613
APCL15_356529E2621
APCL15_355926E2629
APCL15_372981E2637
E
APCL15_095E2550
APCL15_356339E2558
APCL15_362409E2566
APCL15_065E2574
APCL15_076E2582
APCL15_072E2590
APCL15_043E2598
APCL15_034E2606
APCL15_404287E2614
XXXX_E2622
APCL15_369712E2630
APCL15_355605E2638
F
APCL15_372325E2551
APCL15_089E2559
APCL15_068E2567
APCL15_355473E2575
APCL15_407280E2583
APCL15_073E2591
APCL15_354092E2599
APCL15_402943E2607
APCL15_024E2615
APCL15_405197E2623
APCL15_399571E2631
APCL15_371638E2639
G
APCL15_094E2552
APCL15_370033E2560
APCL15_355486E2568
APCL15_354420E2576
APCL15_064E2584
APCL15_354107E2592
APCL15_074E2600
APCL15_354642E2608
APCL15_401342E2616
APCL15_374610E2624
APCL15_403215E2632
APCL15_356440E2640
H
APCL15_355398E2553
APCL15_372651E2561
APCL15_066E2569
APCL15_400408E2577
APCL15_356492E2585
APCL15_069E2593
APCL15_369437E2601
APCL15_369053E2609
APCL15_355704E2617
APCL15_407105E2625
APCL15_026E2633
APCL15_032E2641

Plate_Maps - APCL_E2546-2641.pdf