E1793 - failed to sequence - no gel, low digest concentration, should ligate at 10ng - has not been pulled for redigest, pull for low conce digest

why is column 7 so much fuller than the rest of the wells?

E1872 - failed to sequence  -  no gel, had plenty of DNA,  should ligate at 10ng - has been pulled for redigest

E1862 - failed to sequence  -  no gel, had plenty of DNA,  should ligate at 10ng - has been pulled for redigest

E1797 - failed to sequence  -  no gel, had plenty of DNA,  should ligate at 10ng - has been pulled for redigest


why are the bottoms of some wells green?






Original evernote map from 2015-06-29 Monday:

1
2
3
4
5
6
7
8
9
10
11
12
A
APCL12_118E1783
APCL12_126E1791
APCL12_134E1799
APCL12_142E1807
APCL12_151E1815
APCL12_159E1823
APCL12_167E1831
APCL12_175E1839
APCL12_183E1847
APCL12_191E1855
APCL12_199E1863
APCL12_207E1871
B
APCL12_119E1784
APCL12_127E1792
APCL12_135E1800
APCL12_143E1808
APCL12_152E1816
APCL12_160E1824
APCL12_168E1832
APCL12_176E1840
APCL12_184E1848
APCL12_192E1856
APCL12_200E1864
APCL12_208E1872
C
APCL12_120E1785
APCL12_128E1793
APCL12_136E1801
APCL12_144E1809
APCL12_153E1817
APCL12_161E1825
APCL12_169E1833
APCL12_177E1841
APCL12_185E1849
APCL12_193E1857
APCL12_201E1865
APCL12_209E1873
D
APCL12_121E1786
APCL12_129E1794
APCL12_137E1802
APCL12_145E1810
APCL12_154E1818
APCL12_162E1826
APCL12_170E1834
APCL12_178E1842
APCL12_186E1850
APCL12_194E1858
APCL12_202E1866
APCL12_210E1874
E
APCL12_122E1787
APCL12_130E1795
APCL12_138E1803
APCL12_146E1811
APCL12_155E1819
APCL12_163E1827
APCL12_171E1835
APCL12_179E1843
APCL12_187E1851
APCL12_195E1859
APCL12_203E1867
APCL12_211E1875
F
APCL12_123E1788
APCL12_131E1796
APCL12_139E1804
APCL12_148E1812
APCL12_156E1820
APCL12_164E1828
APCL12_172E1836
APCL12_180E1844
APCL12_188E1852
APCL12_196E1860
APCL12_204E1868
APCL12_212E1876
G
APCL12_124E1789
APCL12_132E1797
APCL12_140E1805
APCL12_149E1813
APCL12_157E1821
APCL12_165E1829
APCL12_173E1837
APCL12_181E1845
APCL12_189E1853
APCL12_197E1861
APCL12_205E1869
APCL12_213E1877
H
APCL12_125E1790
APCL12_133E1798
APCL12_141E1806
APCL12_150E1814
APCL12_158E1822
APCL12_166E1830
APCL12_174E1838
APCL12_182E1846
APCL12_190E1854
APCL12_198E1862
APCL12_206E1870
APCL14_560E1878