2018-08-17 Friday
- cleaned and quantified
2018-08-16 Thursday
- loaded and digested
number of samples
15% error - 10 % didn't work
reaction size
enzyme 1 original conc U/mL
enzyme 2 original conc
pipette master mix into each rxn:
# of units of each enzyme per 30uL rxn
63
72
30
100000
10000
11.999928
12
`
µL sample
enzyme 1
enzyme 2
µL pH20
µL buffer
per sample
18
0.072
0.72
8
3
869
master mix
5
52
595
217
divide final master mix into 8 wells:
109
extr_for_digest_prep.pdf