The majority of this plate failed to sequence - what went wrong?

Could it be failing to vortex plate before pulling for digests?  Forgot to do that again for the redigest on 11/9/2017

Originally digested on 4/4/2016

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2
3
4
5
6
7
8
9
10
11
12
A
APCL15_402499D3000
APCL15_405921D3008
APCL15_375271D3016
APCL15_403055D3024
APCL15_403391D3032
APCL15_401458D3040
APCL15_404313D3048
APCL15_399551D3056
APCL15_406317D3064
APCL15_353379D3072
APCL15_403393D3080
APCL15_405360D3088
B
APCL15_399614D3001
APCL15_407055D3009
APCL15_403102D3017
APCL15_023D3025
APCL15_405904D3033
APCL15_403777D3041
APCL15_025D3049
APCL15_402510D3057
APCL15_404146D3065
XXXX_D3073
APCL15_406108D3081
APCL15_407646D3089
C
APCL15_406164D3002
APCL15_399705D3010
APCL15_404845D3018
APCL15_404371D3026
APCL15_022D3034
APCL15_403889D3042
APCL15_402874D3050
APCL15_407622D3058
APCL15_404829D3066
APCL15_021D3074
APCL15_404653D3082
APCL15_403881D3090
D
APCL15_404545D3003
APCL15_407195D3011
APCL15_405940D3019
APCL15_407223D3027
APCL15_020D3035
APCL15_403655D3043
APCL15_404060D3051
APCL15_402230D3059
APCL15_016D3067
APCL15_404741D3075
APCL15_402921D3083
APCL15_017D3091
E
APCL15_014D3004
APCL15_407137D3012
APCL15_402189D3020
APCL15_404854D3028
XXXX_D3036
APCL15_403879D3044
APCL15_018D3052
APCL15_407028D3060
APCL15_400235D3068
APCL15_403866D3076
APCL15_403566D3084
APCL15_402768D3092
F
APCL15_405523D3005
APCL15_405068D3013
APCL15_002D3021
APCL15_400461D3029
APCL15_003D3037
APCL15_404572D3045
APCL15_375642D3053
APCL15_004D3061
APCL15_400919D3069
APCL15_402340D3077
APCL15_007D3085
APCL15_006D3093
G
APCL15_401656D3006
APCL15_404045D3014
APCL15_009D3022
APCL15_370935D3030
APCL15_403698D3038
APCL15_374493D3046
APCL15_403062D3054
APCL15_404526D3062
APCL15_008D3070
APCL15_405714D3078
APCL15_010D3086
APCL15_401616D3094
H
APCL15_407553D3007
APCL15_405452D3015
APCL15_371294D3023
APCL15_019D3031
APCL15_372491D3039
APCL15_375598D3047
APCL15_371708D3055
APCL15_370907D3063
APCL15_370885D3071
APCL15_373373D3079
APCL15_372956D3087
APCL15_371421D3095